An investigation by the organizers found that the lead singer of Eurovision winners Maneskin did not take drugs during the grand final.
Damiano David, the frontman of the Italian rock band, had tested negative for drugs after the incident on Saturday, according to the European Broadcasting Union.
The band, who won with the song Zitti E Buoni, "strongly denied claims of substance use" that surfaced on social media after David appeared to lean over a table while on camera in the contest's green room.
"Following reports of drug use in the green room of the Eurovision Song Contest Grand Final on Saturday 22 May, the European Broadcasting Union (EBU) has undertaken a comprehensive analysis of the evidence, including reviewing all available videos, as requested by the Italian delegation," the EBU said in a statement.
"Earlier today, the lead singer of the band Maneskin voluntarily underwent a drug test, which yielded a negative result as shown by the EBU." We deem the matter closed because no drugs were used in the green room."
"Alarmed that incorrect speculation leading to false news has overshadowed the spirit and result of the event and unfairly affected the band," the EBU said.
David denied using drugs at a press conference following Maneskin's victory, claiming that he bent over because another band member had a broken glass at their feet.
The EBU later stated that band members had requested that David be drug tested in order to dispel the rumors.
The United Kingdom suffered a humiliating loss when James Newman's song Embers was the only one to receive zero points from the jury and the public, placing last on the leader board.
On Monday, Newman, the younger brother of singer John Newman, thanked fans and said he wanted to "reflect on the positives of this wonderful experience" of the competition, which drew over seven million viewers in the UK for the Saturday night finale.
"The thing about writing songs is that there's no guarantee that a song you think would resonate with people will connect with an audience," he wrote on Instagram.
Liz Truss, the UK's International Trade Secretary, dismissed the notion that the UK's zero points were a "post-Brexit snub" and proposed that the BBC not select potential entrants.
"I think there's a fundamental problem with the way we choose our performances and singers for the Eurovision Song Contest," Ms. Truss told LBC's, Nick Ferrari. "I don't think it's a post-Brexit snub." I believe we need more competition to find the right entrant, and I believe they should be put to more public scrutiny.
"I'm here today at LBC answering listener questions; that's the kind of testing our song contest entrants need to go through."
"Maybe LBC, rather than the BBC, should host it."